a photographic atlas for the microbiology laboratory.pdf

A Photographic Atlas

f o r t h e

Microbiology

L A B O R A T O R Y

4th EDITION

Michael J. Leboffe

San Diego City College

Burton E. Pierce

925 W. Kenyon Ave., Unit 12

Englewood, Colorado 80110

www.morton-pub.com

Book Team

Publisher: Douglas N. Morton

Biology Editor: David Ferguson

Production Manager: Joanne Saliger

Production Assistant: Desiree Coscia

Typography: Ash Street Typecrafters, Inc.

Cover Design: Bob Schram, Bookends, Inc.

This book is dedicated to

Michele Elaine Pierce (1950–2010)

Loving wife, mother, and grandmother,

former student,

and skilled and caring Nurse Practitioner

ISBN: 978-089582-872-9

Library of Congress Control Number: 2010942487

10 9 8 7 6 5 4 3 2 1

stored in a retrieval system, or transmitted, in any form or by any

means, electronic, mechanical, photocopying, recording, or otherwise,

without the prior written permission of the copyright owners.

Printed in the United States of America

Preface

A s my fingers hit the keys of my laptop, I realize that I am, after a long, seemingly endless process,

within days of completing the fourth edition of A Photographic Atlas for the Microbiology

Laboratory . At this stage of a book’s life, a new edition ought to be just a matter of touching up

the previous edition. Or so I thought. But, PAML 4e has presented its share of challenges.

First, and foremost, is the fact that this is the first project (out of three previous Atlas editions, three

editions of Microbiology Laboratory Theory and Application , one edition of Microbiology Laboratory

Theory and Application—Brief Edition , and three editions of Exercises for the Laboratory Manual ) I

have worked on without my longtime friend and colleague, Burt Pierce . Burt made the courageous and

healthy decision to retire and move to Portland, OR, to enjoy life with his wife, three dogs, and two cats

(notice the absence of any microbes in the family). Yet, while he was not an active participant, his influ-

ence remains in this edition. A majority of his written and photographic contributions are still here, and

I have tried to live up to his eye for detail, his demand for excellence, and his dedication to knowing our

readership. His philosophy was that even though we were writing for undergraduate students and not

professionals, there was no excuse for “dumbing down the material”; he had a steadfast faith in the

intelligence of our readers. Long ago, I was impressed by a speech made by a former San Diego City

College president, in which she appealed for teamwork between faculty, staff and administration with

the phrase, “None of us is as good as all of us.” She was right about the college, and it has been equally

applicable to the books Burt and I have co-authored. Our skills complemented one another and the

books were clearly better because of it. But beyond the production of books, and the associated blood,

sweat, and tears, what mattered most was the friendship and satisfaction of a collective “job well done.”

As the old beer commercial said, “(Burt), I love ya, man!”

A second challenge was that the Atlas has slowly accumulated much more text in support of the

photos. When we did the first edition, the Atlas broke the mold at Morton Publishing by including

much more explanatory text for the photos beyond captions. (In fact, the captions were criticized for

not being very informative!) Burt and I maintained that microbiology, by nature, is very different from

other disciplines and required background material; a photo album wouldn’t suffice. While we have

continued to add photos and expand coverage in each edition, the increase in text has considerably

outpaced the photos. (For those of you who have been with us through all four editions, compare

photograph sizes in the first edition to this one!) Writing is not easy for me. But, I’ve finished reading

the proofs and somehow it got written. Chalk up another challenge being met.

In many ways, this edition is like a first edition. Coverage has expanded from being primarily a

book with a medical microbiology emphasis to one with a more balanced emphasis of microbiology in

general. Following is a summary of the major changes in this edition.

The original artwork has been replaced with professional renderings. Many of the older photos

have been replaced with newer ones, and many new photos have been added. Between the artwork

and photos, over 200 new figures (representing approximately 25% of the total) can be found.

Four new chapters have been added. Chapter 1 provides an introduction to microbiology and

presents a perspective on the places Bacteria and Archaea occupy in the biological world. It also

expands the justification for the book’s reorganization (see the following paragraph). Chapter 11

covers some of the most important groups within the Domain Bacteria . Chapters 13 and 14 do the

same for the Domains Archaea and Eukarya , respectively.

Chapters have been resequenced to better reflect the process followed by a working microbiologist,

so isolation techniques and selective media have been moved up to Chapter 2. The chapters that

follow continue the process: growth patterns (Chapter 3), microscopy and staining (Chapters 4, 5,

and 6), biochemical testing (Chapter 7), serological testing (Chapter 8), and molecular techniques

(Chapter 9). The next chapters cover the microbes themselves, beginning with viruses (Chapter 10),

iii

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