Microbiological Media for the Examination of Food, 2nd ed - Ronald M. Atlas.pdf

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HANDBOOK OF
Microbiological
Examination of Food
SECOND EDITION
By
RONALD M. ATLAS
Boca Raton London New York
A CRC title, part of the Taylor & Francis imprint, a member of the
Taylor & Francis Group, the academic division of T&F Informa plc.
Media for the
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Published in 2006 by
CRC Press
Taylor & Francis Group
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Boca Raton, FL 33487-2742
© 2006 by Taylor & Francis Group, LLC
CRC Press is an imprint of Taylor & Francis Group
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Printed in the United States of America on acid-free paper
10987654321
International Standard Book Number-10: 0-8493-3561-2 (Hardcover)
International Standard Book Number-13: 978-0-8493-3561-7 (Hardcover)
Library of Congress Card Number 2005054896
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Library of Congress Cataloging-in-Publication Data
Atlas, Ronald M. 1946-
Handbook of microbiological media for the examination of food / by Ronald M. Atlas.--2nd ed.
p. cm.
Includes bibliographical references and index.
ISBN 0-8493-3561-2 (alk. paper)
1. Food--Microbiology--Laboratory manuals. 2. Culture media (Biology)--Laboratory manuals. I. Title:
Microbiological medica for the examination of food. II. Title.
QR115.A84 2006
664'.001'579--dc22
2005054896
Taylor & Francis Group
is the Academic Division of Informa plc.
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Preface
methods of preparation, and uses of each medium. It
lists the common synonyms for the various media
and the commercial sources when they are available.
The index provides a convenient tool for finding the
media by names and application for the detection of
specific microorganisms of interest.
Increased emphasis on the microbiological safety of
foods has led to greater testing of foods for poten-
tially pathogenic microorganisms. Careful quality
control in the food industry has become essential for
preventing outbreaks of foodborne disease and for
controlling food spoilage. Many foods are routinely
examined for the presence of disease-causing and
food spoilage microorganisms. Many of these qual-
ity control procedures involve the cultivation of
microorganisms using a variety of media that are
described in the second edition of the Handbook of
Microbiological Media for the Examination of Food .
Importantly, the Handbook of Microbiological
Media for the Examination of Food includes
descriptions of expected results as they apply to
microorganisms of importance for the examination
of foods. The compositions of various media can be
compared so that alternate media can be used. Simi-
lar or identical media described by different names
that actually have the same formulations can be
readily identified.
Given that an estimated 14 million cases of food-
borne disease with known microbial pathogens
occur every year in the United States alone, the Food
and Drug Administration and U.S. Department of
Agriculture have been implementing new regula-
tions and guidance for the microbial testing of a
variety of foods. Similarly, Europe and other regions
of the developed world are implementing stricter
oversight of food safety to ensure that foods are free
of potentially dangerous pathogens, e.g., Salmonella
spp, Vibrio spp., Listeria monocytogenes , Campylo-
bacter jejuni , etc.
Emerging diseases caused by foodborne pathogens,
such as Escherichia coli O157:H7, that have high
mortality rates have raised special concerns. This
has encouraged the development of new media for
the cultivation of bacteria, fungi, and viruses that
can aid in the rapid detection of microorganisms in
foods and the protection of the food supply. Many of
these new media include chromogenic or fluoro-
genic substrates that permit the specific rapid detec-
tion of pathogens of concern.
The second edition of the Handbook of Microbiolog-
ical Media for the Examination of Food provides
descriptions of the media used to cultivate microor-
ganisms from foods. Almost 1,400 media are
described in the Handbook , including all the media
recommended by the Food and Drug Administration
for the detection of microorganisms in foods as well
as media used elsewhere in the world. The Hand-
book includes descriptions of the media used to cul-
tivate microorganisms used for food production as
well as those that are important for quality control,
i.e., the cultivation of potential pathogens and food-
spoilage microorganisms.
Using the second edition of the Handbook of Micro-
biological Media for the Examination of Food
should save time and effort for anyone cultivating
microorganisms. The media are organized alphabeti-
cally. Each medium includes the composition,
instructions for the preparation, and uses of each of
the media.
The information needed to prepare media for the
cultivation of microorganisms can easily be found in
the Handbook . It should be a valuable resource for
anyone working in the area of food microbiology.
About the Author
Ronald M. Atlas is Professor of Biology at the Uni-
versity of Louisville. He received his B.S. from the
State University of New York at Stony Brook
in 1968 and his M.S. and Ph.D. from Rutgers in
1970 and 1972, respectively. Dr. Atlas has received
a number of honors including: The University of
Louisville Excellence in Research Award, the
Johnson and Johnson Fellowship for Biology, and
the ASM Award in Applied and Environmental
Microbiology.
He has taught a variety of courses in microbiology
including food and industrial microbiology and has
authored several microbiology textbooks. He has
conducted studies on the use of gene probes and the
polymerase chain reaction for the detection of
pathogens and indicator bacteria in water and food.
He has served on the editorial boards of Applied
and Environmental Microbiology, BioScience, Bio-
techniques, and Journal of Industrial Microbiology .
He is editor of CRC Critical Reviews in Microbiol-
ogy. He also has served as President of the Ameri-
can Society for Microbiology.
The second edition of the Handbook of Microbiolog-
ical Media for the Examination of Food compiles in
one place in a consistent style the formulations,
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